Keith Cook's research interests include: artificial lungs; hemodynamics; pulmonary drug delivery; liquid ventilation; right ventricular function; and critical care medicine.
Destination therapy artificial lungs
Only a small fraction of patients who need a lung transplant will ever be placed on the waiting list. Thus, our group is developing permanent means of respiratory support for patients with chronic lung disease. To achieve this, we need to expand the useful lifetime of artificial lungs to allow permanent, home-based support. Projects in this area include development of compact, highly biocompatible artificial lungs; various means to slow blood clotting within these devices; and biofabricated, tissue-based lungs.
Pulmonary drug delivery using perfluorocarbon emulsions
In many lung diseases, direct delivery of inhaled drugs to the lungs is desired, but the diseased airways are blocked by mucus or inflammatory exudates. To overcome this issue, our group emulsifies drugs within perfluorocarbon liquids (PFCs) and delivers these emulsions to the lungs. When instilled in the lung, PFC emulsions penetrate down to the alveolar level, wash out harmful fluids, open airways, provide normal or even enhanced gas exchange, and hasten lung recovery. Current applications include severe respiratory infections and acute lung injury.
Blood-biomaterial interactions: surface coatings, pharmaceuticals, and modeling
Blood begins to clot instantly whenever it touches an artificial material. Over time, this causes failure of numerous blood bearing medical devices, including catheters, grafts, and various artificial organs. Our group works to develop various approaches to passivate biomaterial surfaces in these devices and thus slow coagulation, but without using systemic anticoagulants that can cause bleeding. We are currently exploring the use of zwitterionic surface coatings, surface nitric oxide flux, and highly selective inhibitors of the intrinsic branch of the coagulation cascade.
2012 - Present
- Schewe RE, Khanafer KM, Orizondo RA, Cook KE. Thoracic artificial lung impedance studies using computational fluid dynamics and in vitro models. Annals of Biomedical Engineering 40: 628-36, 2012.
- Pohlmann JR, Akay B, Camboni D, Koch KL, Mervak BM, Cook KE. A low mortality model of chronic pulmonary hypertension in sheep. Journal of Surgical Research 175, 44–48, 2012.
- Chkourko HS, Guerrero-Serna G, Lin X, Dawish N, Pohlmann JR, Cook KE, Martens JR, Rothenberg E, Mussa H, Delmar M. Remodeling of mechanical junctions and of microtubule-associated proteins accompany cardiac connexin43 lateralization. Heart Rhythm 9: 1133-1140. 2012
- Akay B, Foucher JA, Camboni D, Koch KL, Kawatra A, Cook KE. Hemodynamic design requirements for in series thoracic artificial lung attachment in a model of pulmonary hypertension. ASAIO Journal 58: 426-31, 2012.
- Khanafer K, Cook KE, Marafie A. The role of porous media in modeling fluid flow within hollow fiber membranes of the total artificial lung (TAL). Journal of Porous Media 15: 113-122, 2012.
- Schewe RE, Scipione CN, Koch KL, Cook KE. In-parallel attachment of a low resistance compliant thoracic artificial lung under rest and simulated exercise. Annals of Thoracic Surgery 94: 1688-94, 2012.
- Schewe RE, Khanafer KM, Arab A, Mitchell JA, Skoog DJ, Cook KE. Design and in vitro assessment of a compliant thoracic artificial lung. ASAIO Journal 58: 583–589, 2012.
- Scipione CN, Schewe RE, Koch KL, Shaffer A, Iyengar A, Cook KE. Use of a low resistance compliant thoracic artificial lung in the pulmonary artery to pulmonary artery configuration. Journal of Thoracic and Cardiovascular Surgery 145:1660-6, 2013.
- Amoako KA, Montoya JP, Major TC, Handa H, Brant DO, Suhaib AB, Meyerhoff ME, Bartlett RH, Cook KE. Fabrication and in vivo thrombogenicity testing of nitric oxide generating artificial lungs. Journal of Biomedical Materials Research, Part A 101: 3511–3519, 2013.
- Camboni D, Rojas A, Sassalos P, Spurlock D, Koch KL, Menchak S, Singleton J, Boothman E, Haft JW, Bartlett RH, Cook KE. Long-term animal model of venovenous extracorporeal membrane oxygenation with atrial septal defect as a bridge to lung transplantation. ASAIO Journal 59(6):558-63, 2013.
- Orizondo RA, Babcock CI, Fabiilli ML, Fowlkes JB, Younger JG, Cook KE. Characterization of a reverse phase perfluorocarbon emulsion for the pulmonary delivery of tobramycin. Journal of Aerosolized Medicine and Pulmonary Drug Delivery, 27, 392–399, 2014.
- Biscotti MM, Vail E, Cook KE, Kachulis B, Rosenzweig EB, Bacchetta M. Extracorporeal membrane oxygenation with subclavian artery cannulation in awake patients with pulmonary hypertension. ASAIO Journal, 60(6):748-50, 2014.
- Gupta S, Amoako KA, Suhaib A, Cook KE. Multi-modal, surface focused anticoagulation using poly-2-methoxyethylacrylate polymer grafts and surface nitric oxide release. Advanced Materials Interfaces 1, 140012, 2014.
- Sundaram HS, Han X, Nowinski AK, Brault ND, Li Y, Ella-Menye JR, Amoaka KA, Cook KE, Patrick M, Senecal K, Jiang S. Achieving one-step surface coating of highly hydrophilic poly(carboxybetaine methacrylate) polymers on hydrophobic and hydrophilic surfaces. Advanced Materials Interfaces 1, 140071, 2014.
- Orizondo RA, Fabiilli ML, Morales MA, Cook KE. Effects of emulsion composition on pulmonary tobramycin delivery during antibacterial perfluorocarbon ventilation. Journal of Aerosolized Medicine and Pulmonary Drug Delivery 29:251-9, 2016.
- Amoako KA, Suhaib A, Sundaram HS, Jiang S, Cook KE. Multimodal, Biomaterial-Focused Anticoagulation Via Super-low Fouling Zwitterionic Functional Groups Coupled with Anti-Platelet Nitric Oxide Release. Advanced Materials Interfaces 3(6), 1500646, 2016.
- Hong D, Hung H, Wu K, Lin X, Sun F, Zhang P, Liu S, Cook KE, Jiang S. Achieving ultralow fouling under ambient conditions via Si-ARGET ATRP of carboxybetaine. ACS Appl. Mater. Interfaces, 2017, 9 (11), 9255–9259.
- Skoog DJ, Scipione CN, Pohlmann JR, Demos DS, Iyengar A, Schewe RE, Koch KL, Suhaib AB, Cook KE. 14 Day in vivo testing of a compliant thoracic artificial lung, ASAIO Journal 63:644–649, 2017.
2007 - 2011
- Sato H, Griffith GW, Hall CM, Toomasian JM, Hirschl RB, Bartlett RH, Cook KE. Seven day artificial lung testing in an in-parallel configuration. Annals of Thoracic Surgery 84: 988-994, 2007.
- Sato H, Hall CM, Lafayette NG, Pohlmann J, Padiyar N, Toomasian JM, Haft JW, Cook KE. Thirty-day, in parallel artificial lung testing in sheep. Annals of Thoracic Surgery 84: 1136-1143, 2007.
- Zierenberg JR, Fujioka H, Cook KE, Grotberg JB. Pulsatile flow and oxygen transport past cylindrical fiber arrays for an artificial lung: computational and experimental studies. Journal of Biomechanical Engineering 130, 31019-1-31019-12, 2008.
- Kuo AS, Perlman CE, Mockros LF, Cook KE. Pulmonic valve function during thoracic artificial lung attachment. ASAIO Journal 54: 197-202, 2008.
- Sato H, Hall CM, Griffith GW, Johnson KF, Mcgillicuddy JW, Bartlett RH, Cook KE. Large animal model of chronic pulmonary hypertension. ASAIO Journal 54, 396-400, 2008.
- Xu H, Reynolds MM, Cook KE, Toscano JP. 2-hydroxy-5-nitrobenzyl as a diazeniumdiolate protecting group: application in NO-releasing polymers with enhanced biocompatibility. Organic Letters 10: 4593–4596, 2008.
- Kim J, Sato H, Griffith GW, Cook KE. Cardiac output during high afterload artificial lung attachment. ASAIO Journal, ASAIO Journal 55: 73–77, 2009.
- Reoma JL, Rojas A, Kim AC, Khouri JS, Boothman E, Brown K, Grotberg J, Cook KE, Bartlett RH, Hirschl RB, Mychaliska GB. Development of an artificial placenta I: pumpless AV-ECLS in a neonatal sheep model, Journal of Pediatric Surgery 44: 53-9, 2009.
- LaFayette NG, Schewe RE, Montoya PJ, Cook KE. Performance of a Medarray silicone hollow fiber oxygenator. ASAIO Journal 55: 382-387, 2009.
- Reoma JL, Rojas A, Krause E, Obeid NR, Lafayette N, Cook KE, Punch JD, Bartlett RH. Lung physiology during ECMO resuscitation of DCD donors followed by in-vivo assessment of lung function. ASAIO Journal 55: 388-394, 2009.
- Pohlmann JR, Hampton C, Toomasian JM, Romeo A, Cook KE, Bartlett RH. The relationships between air exposure, negative pressure, and hemolysis. ASAIO Journal 55: 469-473, 2009.
- Obeid NR, Rojas A, Reoma JL, Cook KE, Bartlett RH, Punch JD. Organ donation after cardiac determination of death (DCDD): a swine model. ASAIO Journal 55:562–568, 2009.
- Kuo AS, Sato H, Reoma JL, Cook KE. Pulmonary system impedance and right ventricular function. Cardiovascular Engineering 9:153–160, 2009.
- Rojas A, Reoma JL, Krause E, Cook KE, Bartlett RH, Punch JD. Extracorporeal support improves donor renal graft function after cardiac death. American Journal of Transplantation 10: 1365-1374, 2010.
- Akay B, Reoma JL, Camboni D, Pohlmann JR, Albert JM, Kawatra A, Gouch AD, Bartlett RH, Cook KE. In parallel artificial lung attachment at high flows in normal and pulmonary hypertension models. Annals of Thoracic Surgery 90: 259–65, 2010.
- Pohlmann JR, Brant DO, Daul MA, Reoma JL, Kim AC, Johnson KJ, Bartlett RH, Cook KE, Hirschl RB. Total liquid ventilation provides superior respiratory support to conventional mechanical ventilation in a large animal model of severe respiratory failure. ASAIO Journal, 57:1-8, 2011.
- Camboni D, Akay B, Sassalos P, Toomasian JM, Haft JW, Bartlett RH, Cook KE. Use of venovenous extracorporeal membrane oxygenation and an atrial septostomy for pulmonary and right ventricular failure. Annals of Thoracic Surgery 91:144-149, 2011.
- Camboni D, Akay B, Pohlmann JR, Koch KL, Haft JW, Bartlett RH, Cook KE. Veno-venous extracorporeal membrane oxygenation with interatrial shunting: A novel approach to lung transplantation for patients in right ventricular failure. Journal of Thoracic and Cardiovascular Surgery 141:537-42, 2011.
- Rojas-Pena A, Koch KL, Hall CM, Bergin IL, Cook KE. Quantification of thermal spread and burst pressure during endoscopic vessel harvesting (EVH). Journal of Thoracic and Cardiovascular Surgery, 142: 203-208, 2011.
- Rojas A, Hall CM, Cook KE, Bartlett RH, Arenas JD, Punch JD. Timing of heparin and reperfusion temperature during procurement of organs with extracorporeal support in donors after circulatory determination of death. ASAIO Journal 57:368-74, 2011.
- Amoako KA, Cook KE. Nitric oxide-generating silicone as a blood-contacting biomaterial. ASAIO Journal, 57:539–544, 2011.
Prior to 2007
- Vaslef SN, Cook KE, Leonard RJ, Mockros LF, Anderson RW. Design and evaluation of a new, low pressure loss, implantable artificial lung. ASAIO Journal 40: M522-M526, 1994.
- Vaslef SN, Mockros LF, Cook KE, Leonard RJ, Sung JC, Anderson RW. Computer-assisted design of an implantable intrathoracic artificial lung. Artificial Organs 18: 813-817, 1994.
- Cook KE, Makarewicz AJ, Backer CL, Mockros LF, Przybylo HJ, Crawford S.E., Leonard RJ, Mavroudis C. Testing of an intrathoracic artificial lung in a pig model. ASAIO Journal 42: M604-M609, 1996.
- Hocking LM, Debler WR, Cook KE. The growth of leading-edge distortions on a viscous sheet. Physics of Fluids 11: 307-313, 1999.
- Boschetti F, Perlman CE, Cook KE, Mockros LF. Hemodynamic effects of attachment modes and device design of a thoracic artificial lung. ASAIO Journal 46: 42-48, 2000.
- Zwischenberger JB, Anderson CM, Cook KE, Lick SD, Mockros LF, Bartlett RH. Development of an artificial lung: challenges and progress. ASAIO Journal 47: 316-20, 2001.
- Dodge-Khatami A, Backer CL, Holinger LD, Mavroudis C, Cook KE, Crawford SE. Healing of a free tracheal autograft is enhanced by topical VEGF in an experimental rabbit model. Journal of Thoracic and Cardiovascular Surgery 122: 554-561, 2001.
- Cook KE, Maxhimer J, Leonard DJ, Mavroudis C, Backer CL, Mockros LF. Platelet and leukocyte activation and design consequences for thoracic artificial lungs. ASAIO Journal 48: 620-630, 2002.
- Zias EA, Mavroudis C, Cook KE, Makarewicz AJ, Backer CL, Hernandez JM. The effect of pulmonary circulation hemodynamics on right ventricular unloading via the bidirectional Glenn shunt: implications for congenitally corrected transposition repair. Seminars in Thoracic & Cardiovascular Surgery. Pediatric Cardiac Surgery Annual 6:27-32, 2003.
- Boschetti F, Cook KE, Perlman CE, Mockros LF. Blood flow pulsatility effects on oxygen transfer in artificial lungs. ASAIO Journal 49: 678-686, 2003.
- Griffith GW, Toomasian JM, Schreiner RJ, Dusset CM, Cook KE, Osterholtzer KR, Merz SI, Bartlett RH. Hematologic changes during short term tidal flow ECLS. Perfusion 19: 359-363, 2004.
- Toomasian JM, Schreiner RJ, Griffith GW, Meyers DE, Schmidt ME, Hagan SE, Bartlett RH, Cook KE.
- A polymethylpentene fiber gas exchanger for long-term extracorporeal life support. ASAIO Journal, 51: 390-397, 2005.
- Cook KE, Perlman CE, Seipelt R, Backer CL, Mavroudis C, Mockros LF. Hemodynamic and gas transfer properties of a compliant thoracic artificial lung. ASAIO Journal, 51: 404-411, 2005.
- Perlman CE, Cook KE, Seipelt R, Mavroudis C, Backer CL, Mockros LF. Hemodynamic consequences of artificial lung attachment in an in vivo porcine model, ASAIO Journal, 51: 412-425, 2005.
- Carroll CL, Backer CL, Mavroudis C, Cook KE, Goodman DM. Inhaled prostacyclin following surgical repair of congenital heart disease – a pilot study. Journal of Cardiac Surgery 20: 436-439, 2005.
- McGillicuddy JW, Chambers SD, Galligan DT, Hirschl RB, Bartlett RH, Cook KE. In vitro, fluid mechanical effects of thoracic artificial lung compliance. ASAIO Journal 51: 789-794, 2005.
- Sato H, McGillicuddy JW, Griffith GW, Cosnowski AM, Chambers SD, Hirschl RB, Bartlett RH, Cook KE. Effects of artificial lung compliance on in vivo pulmonary system hemodynamics. ASAIO Journal 52: 248-256, 2006.
- Water in Perfluorocarbon Emulsions For Intrapulmonary Drug Delivery, Pattent Application Filed
...in with the good
With a grant from the U.S. Army, Biomedical Engineering professor Keith Cook is revolutionizing the field of external oxygenators.
A breath of fresh perfluorocarbon
Many chronic lung diseases limit the ability of inhaled medications to reach the areas they need to reach. BME’s Diane Nelson has been working on a solution: liquid perfluorocarbon emulsions.
Cook discusses artificial lung technology on SciTechNow
In an interview with SciTechNow, Biomedical Engineering Professor Keith Cook discussed advances in artificial lung use and technology.
Cook featured in New Scientist for artificial lung research
BME’s Keith Cook was featured in a New Scientist article on the development of artificial lungs.