Cook Cardiopulmonary Engineering Group

cook-lab-banner.jpgKeith Cook's research focuses on applying engineering to critical care medicine. This research melds mechanical, chemical, and material science concepts toward the development of artificial and tissue-based lungs, pulmonary drug delivery, and the computational modeling and prevention of coagulation in medical devices.

Faculty

Keith Cook

Keith Cook

Professor of Biomedical Engineering

Current research projects in his group include thoracic artificial lungs, perfluorocarbon emulsions for pulmonary drug delivery, new biomaterial approaches for reducing coagulation at artificial surfaces, and PET-based diagnostic tools for right ventricular dysfunction. Of note, his laboratory was the first to produce 24 hour, week, and 30-day in vivo studies of thoracic artificial lungs, and his group is working on a artificial lung intended as destination therapy for years of respiratory support. Professor Cook currently serves as an editor of the American Society of Artificial Internal Organs Journal.
Office
Scott Hall
Phone
412.268.7383
Fax
412.268.3025
Email
keicook@andrew.cmu.edu
Google Scholar
Keith Cook
Curriculum Vitae
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Projects

Keith Cook's research interests include: artificial lungs; hemodynamics; pulmonary drug delivery; liquid ventilation; right ventricular function; and critical care medicine.

Research team

Neil Carleton

Neil Carleton

Lab Manager

Research interests
Assisting with in vivo surgeries and post-operative care; developing in vitro methods for evaluating antibacterial properties of nitric oxide and copper nanoparticles in artificial lung setting
Email
ncarleton42@gmail.com
Saif Al-Qatarneh

Saif Al-Qatarneh

Post-Doctorate

Research interests
Optimizing mechanical ventilation techniques for in vivo pulmonary drug delivery using perfluorocarbon emulsions.
Email
Saif.AlQatarneh@chp.edu
Erica Comber

Erica Comber

Doctorate

Research interests
Tissue engineering artificial lungs, minimizing host-reactions to implants, and promoting angiogenesis at the site of injury. Outside of research, interests in: providing affordable, assistive medical devices for individuals with mobility disabilities.
Email
ecomber@andrew.cmu.edu
Angela Lai

Angela Lai

Doctorate

Research interests
Destination therapy artificial lungs; in vitro and in vivo evaluation of nitric oxide and copper nanoparticles to prevent clot formation and infection.
Email
angelala@andrew.cmu.edu
Noritsugu Naito

Noritsugu Naito

Post-Doctorate

Research interests
In vivo evaluation of nitric oxide-generating, surface coated artificial lung.
Email
nnaito@andrew.cmu.edu
Diane Nelson

Diane Nelson

Doctorate

Research interests
Optimizing pulmonary drug delivery using perfluorocarbon emulsions.
Email
dlnelson@andrew.cmu.edu
Rei Ukita

Rei Ukita

Doctorate

Research interests
Biomaterial and biochemical approaches to reduce blood coagulation in an artificial lung settin, and the various methods of evaluating such approaches; applying low-fouling zwitterionic polymer coatings and nitric oxide surface flux to artificial lung gas fibers to reduce protein adsorption and platelet activation; using animal models and bench top blood-contact experiments to evaluate these different strategies. Ultimately, these different approaches can be combined to extend the longevity of artificial lungs, which can then be used as destination therapy for chronic lung disease patients.
Email
rukita@andrew.cmu.edu

Publications

2012 - Present
  • Schewe RE, Khanafer KM, Orizondo RA, Cook KE. Thoracic artificial lung impedance studies using computational fluid dynamics and in vitro models. Annals of Biomedical Engineering 40: 628-36, 2012.
  • Pohlmann JR, Akay B, Camboni D, Koch KL, Mervak BM, Cook KE. A low mortality model of chronic pulmonary hypertension in sheep. Journal of Surgical Research 175, 44–48, 2012.
  • Chkourko HS, Guerrero-Serna G, Lin X, Dawish N, Pohlmann JR, Cook KE, Martens JR, Rothenberg E, Mussa H, Delmar M. Remodeling of mechanical junctions and of microtubule-associated proteins accompany cardiac connexin43 lateralization. Heart Rhythm 9: 1133-1140. 2012
  • Akay B, Foucher JA, Camboni D, Koch KL, Kawatra A, Cook KE. Hemodynamic design requirements for in series thoracic artificial lung attachment in a model of pulmonary hypertension. ASAIO Journal 58: 426-31, 2012.
  • Khanafer K, Cook KE, Marafie A. The role of porous media in modeling fluid flow within hollow fiber membranes of the total artificial lung (TAL). Journal of Porous Media 15: 113-122, 2012.
  • Schewe RE, Scipione CN, Koch KL, Cook KE. In-parallel attachment of a low resistance compliant thoracic artificial lung under rest and simulated exercise. Annals of Thoracic Surgery 94: 1688-94, 2012.
  • Schewe RE, Khanafer KM, Arab A, Mitchell JA, Skoog DJ, Cook KE. Design and in vitro assessment of a compliant thoracic artificial lung. ASAIO Journal 58: 583–589, 2012.
  • Scipione CN, Schewe RE, Koch KL, Shaffer A, Iyengar A, Cook KE. Use of a low resistance compliant thoracic artificial lung in the pulmonary artery to pulmonary artery configuration. Journal of Thoracic and Cardiovascular Surgery 145:1660-6, 2013.
  • Amoako KA, Montoya JP, Major TC, Handa H, Brant DO, Suhaib AB, Meyerhoff ME, Bartlett RH, Cook KE. Fabrication and in vivo thrombogenicity testing of nitric oxide generating artificial lungs. Journal of Biomedical Materials Research, Part A 101: 3511–3519, 2013.
  • Camboni D, Rojas A, Sassalos P, Spurlock D, Koch KL, Menchak S, Singleton J, Boothman E, Haft JW, Bartlett RH, Cook KE. Long-term animal model of venovenous extracorporeal membrane oxygenation with atrial septal defect as a bridge to lung transplantation. ASAIO Journal 59(6):558-63, 2013.
  • Orizondo RA, Babcock CI, Fabiilli ML, Fowlkes JB, Younger JG, Cook KE. Characterization of a reverse phase perfluorocarbon emulsion for the pulmonary delivery of tobramycin. Journal of Aerosolized Medicine and Pulmonary Drug Delivery, 27, 392–399, 2014.
  • Biscotti MM, Vail E, Cook KE, Kachulis B, Rosenzweig EB, Bacchetta M. Extracorporeal membrane oxygenation with subclavian artery cannulation in awake patients with pulmonary hypertension. ASAIO Journal, 60(6):748-50, 2014.
  • Gupta S, Amoako KA, Suhaib A, Cook KE. Multi-modal, surface focused anticoagulation using poly-2-methoxyethylacrylate polymer grafts and surface nitric oxide release. Advanced Materials Interfaces 1, 140012, 2014.
  • Sundaram HS, Han X, Nowinski AK, Brault ND, Li Y, Ella-Menye JR, Amoaka KA, Cook KE, Patrick M, Senecal K, Jiang S. Achieving one-step surface coating of highly hydrophilic poly(carboxybetaine methacrylate) polymers on hydrophobic and hydrophilic surfaces. Advanced Materials Interfaces 1, 140071, 2014.
  • Orizondo RA, Fabiilli ML, Morales MA, Cook KE. Effects of emulsion composition on pulmonary tobramycin delivery during antibacterial perfluorocarbon ventilation. Journal of Aerosolized Medicine and Pulmonary Drug Delivery 29:251-9, 2016.
  • Amoako KA, Suhaib A, Sundaram HS, Jiang S, Cook KE. Multimodal, Biomaterial-Focused Anticoagulation Via Super-low Fouling Zwitterionic Functional Groups Coupled with Anti-Platelet Nitric Oxide Release. Advanced Materials Interfaces 3(6), 1500646, 2016.
  • Hong D, Hung H, Wu K, Lin X, Sun F, Zhang P, Liu S, Cook KE, Jiang S. Achieving ultralow fouling under ambient conditions via Si-ARGET ATRP of carboxybetaine. ACS Appl. Mater. Interfaces, 2017, 9 (11), 9255–9259.
  • Skoog DJ, Scipione CN, Pohlmann JR, Demos DS, Iyengar A, Schewe RE, Koch KL, Suhaib AB, Cook KE. 14 Day in vivo testing of a compliant thoracic artificial lung, ASAIO Journal 63:644–649, 2017.
2007 - 2011
  • Sato H, Griffith GW, Hall CM, Toomasian JM, Hirschl RB, Bartlett RH, Cook KE. Seven day artificial lung testing in an in-parallel configuration. Annals of Thoracic Surgery 84: 988-994, 2007.
  • Sato H, Hall CM, Lafayette NG, Pohlmann J, Padiyar N, Toomasian JM, Haft JW, Cook KE. Thirty-day, in parallel artificial lung testing in sheep. Annals of Thoracic Surgery 84: 1136-1143, 2007.
  • Zierenberg JR, Fujioka H, Cook KE, Grotberg JB. Pulsatile flow and oxygen transport past cylindrical fiber arrays for an artificial lung: computational and experimental studies. Journal of Biomechanical Engineering 130, 31019-1-31019-12, 2008.
  • Kuo AS, Perlman CE, Mockros LF, Cook KE. Pulmonic valve function during thoracic artificial lung attachment. ASAIO Journal 54: 197-202, 2008.
  • Sato H, Hall CM, Griffith GW, Johnson KF, Mcgillicuddy JW, Bartlett RH, Cook KE. Large animal model of chronic pulmonary hypertension. ASAIO Journal 54, 396-400, 2008.
  • Xu H, Reynolds MM, Cook KE, Toscano JP. 2-hydroxy-5-nitrobenzyl as a diazeniumdiolate protecting group: application in NO-releasing polymers with enhanced biocompatibility. Organic Letters 10: 4593–4596, 2008.
  • Kim J, Sato H, Griffith GW, Cook KE. Cardiac output during high afterload artificial lung attachment. ASAIO Journal, ASAIO Journal 55: 73–77, 2009.
  • Reoma JL, Rojas A, Kim AC, Khouri JS, Boothman E, Brown K, Grotberg J, Cook KE, Bartlett RH, Hirschl RB, Mychaliska GB. Development of an artificial placenta I: pumpless AV-ECLS in a neonatal sheep model, Journal of Pediatric Surgery 44: 53-9, 2009.
  • LaFayette NG, Schewe RE, Montoya PJ, Cook KE. Performance of a Medarray silicone hollow fiber oxygenator. ASAIO Journal 55: 382-387, 2009.
  • Reoma JL, Rojas A, Krause E, Obeid NR, Lafayette N, Cook KE, Punch JD, Bartlett RH. Lung physiology during ECMO resuscitation of DCD donors followed by in-vivo assessment of lung function. ASAIO Journal 55: 388-394, 2009.
  • Pohlmann JR, Hampton C, Toomasian JM, Romeo A, Cook KE, Bartlett RH. The relationships between air exposure, negative pressure, and hemolysis. ASAIO Journal 55: 469-473, 2009.
  • Obeid NR, Rojas A, Reoma JL, Cook KE, Bartlett RH, Punch JD. Organ donation after cardiac determination of death (DCDD): a swine model. ASAIO Journal 55:562–568, 2009.
  • Kuo AS, Sato H, Reoma JL, Cook KE. Pulmonary system impedance and right ventricular function. Cardiovascular Engineering 9:153–160, 2009.
  • Rojas A, Reoma JL, Krause E, Cook KE, Bartlett RH, Punch JD. Extracorporeal support improves donor renal graft function after cardiac death. American Journal of Transplantation 10: 1365-1374, 2010.
  • Akay B, Reoma JL, Camboni D, Pohlmann JR, Albert JM, Kawatra A, Gouch AD, Bartlett RH, Cook KE. In parallel artificial lung attachment at high flows in normal and pulmonary hypertension models. Annals of Thoracic Surgery 90: 259–65, 2010.
  • Pohlmann JR, Brant DO, Daul MA, Reoma JL, Kim AC, Johnson KJ, Bartlett RH, Cook KE, Hirschl RB. Total liquid ventilation provides superior respiratory support to conventional mechanical ventilation in a large animal model of severe respiratory failure. ASAIO Journal, 57:1-8, 2011.
  • Camboni D, Akay B, Sassalos P, Toomasian JM, Haft JW, Bartlett RH, Cook KE. Use of venovenous extracorporeal membrane oxygenation and an atrial septostomy for pulmonary and right ventricular failure. Annals of Thoracic Surgery 91:144-149, 2011.
  • Camboni D, Akay B, Pohlmann JR, Koch KL, Haft JW, Bartlett RH, Cook KE. Veno-venous extracorporeal membrane oxygenation with interatrial shunting: A novel approach to lung transplantation for patients in right ventricular failure. Journal of Thoracic and Cardiovascular Surgery 141:537-42, 2011.
  • Rojas-Pena A, Koch KL, Hall CM, Bergin IL, Cook KE. Quantification of thermal spread and burst pressure during endoscopic vessel harvesting (EVH). Journal of Thoracic and Cardiovascular Surgery, 142: 203-208, 2011. 
  • Rojas A, Hall CM, Cook KE, Bartlett RH, Arenas JD, Punch JD. Timing of heparin and reperfusion temperature during procurement of organs with extracorporeal support in donors after circulatory determination of death. ASAIO Journal 57:368-74, 2011.
  • Amoako KA, Cook KE. Nitric oxide-generating silicone as a blood-contacting biomaterial. ASAIO Journal, 57:539–544, 2011.
Prior to 2007
  • Vaslef SN, Cook KE, Leonard RJ, Mockros LF, Anderson RW. Design and evaluation of a new, low pressure loss, implantable artificial lung. ASAIO Journal 40: M522-M526, 1994.
  • Vaslef SN, Mockros LF, Cook KE, Leonard RJ, Sung JC, Anderson RW. Computer-assisted design of an implantable intrathoracic artificial lung. Artificial Organs 18: 813-817, 1994.
  • Cook KE, Makarewicz AJ, Backer CL, Mockros LF, Przybylo HJ, Crawford S.E., Leonard RJ, Mavroudis C. Testing of an intrathoracic artificial lung in a pig model. ASAIO Journal 42: M604-M609, 1996.
  • Hocking LM, Debler WR, Cook KE. The growth of leading-edge distortions on a viscous sheet. Physics of Fluids 11: 307-313, 1999.
  • Boschetti F, Perlman CE, Cook KE, Mockros LF. Hemodynamic effects of attachment modes and device design of a thoracic artificial lung. ASAIO Journal 46: 42-48, 2000.
  • Zwischenberger JB, Anderson CM, Cook KE, Lick SD, Mockros LF, Bartlett RH. Development of an artificial lung: challenges and progress. ASAIO Journal 47: 316-20, 2001.
  • Dodge-Khatami A, Backer CL, Holinger LD, Mavroudis C, Cook KE, Crawford SE. Healing of a free tracheal autograft is enhanced by topical VEGF in an experimental rabbit model. Journal of Thoracic and Cardiovascular Surgery 122: 554-561, 2001.
  • Cook KE, Maxhimer J, Leonard DJ, Mavroudis C, Backer CL, Mockros LF. Platelet and leukocyte activation and design consequences for thoracic artificial lungs. ASAIO Journal 48: 620-630, 2002.
  • Zias EA, Mavroudis C, Cook KE, Makarewicz AJ, Backer CL, Hernandez JM. The effect of pulmonary circulation hemodynamics on right ventricular unloading via the bidirectional Glenn shunt: implications for congenitally corrected transposition repair. Seminars in Thoracic & Cardiovascular Surgery. Pediatric Cardiac Surgery Annual 6:27-32, 2003.
  • Boschetti F, Cook KE, Perlman CE, Mockros LF. Blood flow pulsatility effects on oxygen transfer in artificial lungs. ASAIO Journal 49: 678-686, 2003.
  • Griffith GW, Toomasian JM, Schreiner RJ, Dusset CM, Cook KE, Osterholtzer KR, Merz SI, Bartlett RH. Hematologic changes during short term tidal flow ECLS. Perfusion 19: 359-363, 2004.
  • Toomasian JM, Schreiner RJ, Griffith GW, Meyers DE, Schmidt ME, Hagan SE, Bartlett RH, Cook KE
  • A polymethylpentene fiber gas exchanger for long-term extracorporeal life support. ASAIO Journal, 51: 390-397, 2005.
  • Cook KE, Perlman CE, Seipelt R, Backer CL, Mavroudis C, Mockros LF. Hemodynamic and gas transfer properties of a compliant thoracic artificial lung. ASAIO Journal, 51: 404-411, 2005.
  • Perlman CE, Cook KE, Seipelt R, Mavroudis C, Backer CL, Mockros LF. Hemodynamic consequences of artificial lung attachment in an in vivo porcine model, ASAIO Journal, 51: 412-425, 2005.
  • Carroll CL, Backer CL, Mavroudis C, Cook KE, Goodman DM. Inhaled prostacyclin following surgical repair of congenital heart disease – a pilot study. Journal of Cardiac Surgery 20: 436-439, 2005.
  • McGillicuddy JW, Chambers SD, Galligan DT, Hirschl RB, Bartlett RH, Cook KE. In vitro, fluid mechanical effects of thoracic artificial lung compliance. ASAIO Journal 51: 789-794, 2005.
  • Sato H, McGillicuddy JW, Griffith GW, Cosnowski AM, Chambers SD, Hirschl RB, Bartlett RH, Cook KE. Effects of artificial lung compliance on in vivo pulmonary system hemodynamics. ASAIO Journal 52: 248-256, 2006.

Patents

  • Water in Perfluorocarbon Emulsions For Intrapulmonary Drug Delivery, Pattent Application Filed

Media mentions


CMU Engineering

...in with the good

With a grant from the U.S. Army, Biomedical Engineering professor Keith Cook is revolutionizing the field of external oxygenators.

CMU Engineering

A breath of fresh perfluorocarbon

Many chronic lung diseases limit the ability of inhaled medications to reach the areas they need to reach. BME’s Diane Nelson has been working on a solution: liquid perfluorocarbon emulsions.

CMU Engineering

Cook discusses artificial lung technology on SciTechNow

In an interview with SciTechNow, Biomedical Engineering Professor Keith Cook discussed advances in artificial lung use and technology.

CMU Engineering

Department news

Stay up-to-date with each department of Carnegie Mellon’s College of Engineering.

New Scientist

Cook featured in New Scientist for artificial lung research

BME’s Keith Cook was featured in a New Scientist article on the development of artificial lungs.

CMU Engineering

Dowd Seed Fund for Fellowships

Some of the greatest innovations of our time started out with the riskiest ideas. But risky ideas often go unfunded—until someone is brave enough to trust in the researcher's dreams.