Biomedical Engineering

Intracellular trafficking of naked DNA

February 28, 2017

4:30 p.m. ET

Doherty Hall A302

Fan Yuan, Ph.D.
Professor
Department of Biomedical Engineering
Duke University

Abstract

Electrotransfection is a technique for gene delivery. It is also called electroporation in the literature. Compared to viral delivery systems, it is safe, easy to use, and not limited by the size and the number of genes to be delivered simultaneously into a cell. To improve the efficiency of electrotransfection, we have investigated mechanisms of uptake and intracellular transport of plasmid DNA. Results from our studies show that pulsed electrical field stimulates endocytosis, and that electrotransfection involves vesicular transport of DNA through multiple pathways of endocytosis. The findings will be used to develop new strategies for improving gene delivery both in vitro and in vivo.

Coffee and snacks are served at 4:00 P.M. in Scott Hall BME kitchen area, where students may meet the speaker.

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